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Labrador/UCLA Virology Laboratory
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Covalent silver has been successfully tested at the UCLA Medical Labs where it eliminated every virus on which it was tested. Laboratory tests in 1988 by Larry C. Ford, M.D., UCLA School of Medicine, and other researchers have shown that bacteria, viruses and even some fungi cannot live more than six minutes in contact with silver in its metallic form. Many other pathogens are destroyed because the electric charge on the silver particles cause their internal protoplast to collapse, and still others are rendered unable to reproduce.
Covalent Silver (BA-273/CMV3) treatment of Normal PHA Stimulated Peripheral Blood Lymphocytes Infected with HIV_1 JRCFS. Acquired Human Immune-Deficiency Syndrome (AIDS) is a disease which targets and depletes the body's T helper cells. It is caused by the Human Immunodeficiency Virus type 1 (HIV_1). Viral load, as measured in Peripheral Blood Mononuclear Cells (PBMCs) cultures, have been shown to correlate with early infection progression and loss of CD4 cells. Similarly suppression of viral replication by an antiretroviral agent in Vitro is a clear indication that such an agent could be a potential candidate for treatment of HIV_1 infection.
In this experiment, PHA stimulated PBLs infected with HIV_1 JRCSF were treated with BA-273 agent. Supernatants were harvested on day 4 and day 7 tested for P24, to evaluate the inhibitory effect of BA-273 agent on the virus. Peripheral Blood Lymphocytes from a normal donor were stimulated in a PHA containing medium for 72 hours. Cells were washed in RPMI 1640 serum free, re-suspended in a growth medium (RPMI+20% PBS and 10 units/ml IL_2). Cells were counted.
30 million cells were inoculated with HIV_1 JRCSF, at a dose of 10ng P24 virus/ 10 million cells. Add 15ul polybrene. Incubate at 37 degrees Celsius, for two hours.
Wash 2* in serum-free RPMI Re-suspend in growth medium at a density of one million cells/ml. Distribute in a 24-well plate: 1 ml cell suspension per well. Add BA-273 agent at different concentrations in triplicate wells. Use the first three wells as controls.
On day 4, culture was microscopically observed, the cells all looked healthy. There appeared to be no negative reaction due to the addition of BA-273 at any concentration: 10ul, 20ul, 40ul, 80ul, 160ul, or 200ul. Cells in control wells looked no different from those in treated well, indicating a positive dose response. BA- 273 has not affected the conditions of the cells. On day 7, cells were microscopically examined again and similar observations were made as on day 4. (covalent silver had successfully destroyed 30 million infected cultures, most of which were HIV).
P24 RESULTS in ng/ml
BA-273 CONCENTRATE WELL 1 WELL 2 WELL 3
10ul
DAY 4 3.111 3.417 4.625
DAY 7 KILL KILL KILL
20ul
DAY 4 5.332 4.712 3.671
DAY 7 KILL KILL KILL
40ul
DAY 4 3.496 3.309 3.787
DAY 7 KILL KILL KILL
80ul
DAY 4 3.930 5.512 4.495
DAY 7 KILL KILL KILL
160ul
DAY 4 2.418 2.011 1.957
DAY 7 KILL KILL KILL
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